4KTA
Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.065, 60.624, 37.919 |
| Unit cell angles | 90.00, 95.11, 90.00 |
Refinement procedure
| Resolution | 32.080 - 1.950 |
| R-factor | 0.1754 |
| Rwork | 0.173 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.862 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.053 | 0.391 |
| Number of reflections | 10182 | |
| <I/σ(I)> | 14.56 | 2.54 |
| Completeness [%] | 99.0 | 95.7 |
| Redundancy | 7.65 | 4.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 29% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
