4KSO
Crystal Structure of Circadian clock protein KaiB from S.Elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2012-12-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.821, 116.124, 53.228 |
Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
Resolution | 29.689 - 2.622 |
R-factor | 0.2809 |
Rwork | 0.279 |
R-free | 0.31040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qke |
RMSD bond length | 0.004 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.060 | 0.267 |
Number of reflections | 12670 | |
<I/σ(I)> | 14.6 | 2.1 |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.7 | 292 | KaiB were grown from droplets containing 6.5 mg/mL KaiB-KaiC complex, 20 mM Tris (pH 7.8), 100 mM NaCl, 5 mM MgCl2,1 mM ATP and 2 mM BME. The reservoir solution was 100 mM sodium acetate, 500 mM sodium formate and 5% glycerol (v/v), VAPOR DIFFUSION, temperature 292K |