4KSJ

Crystal structure of the OTU domain of Gumby/Fam105B at 1.6 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	43.350, 71.820, 94.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.376 - 1.600
R-factor	0.18215
Rwork	0.180
R-free	0.21547
Structure solution method	SAD
RMSD bond length	0.030
RMSD bond angle	2.201
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.376	1.720	1.660
High resolution limit [Å]	1.600	1.660	1.600
Rmerge	0.061	0.416	0.584
Number of reflections	36961
<I/σ(I)>	36.6	4.5	3.1
Completeness [%]	99.9	100	100
Redundancy	3.8	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		100mM MES, 200mM MgCl2, 19% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP