4KRZ
Apo crystal structure of pyruvate kinase (PYK) from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.785, 121.415, 97.160 |
| Unit cell angles | 90.00, 115.74, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.500 |
| R-factor | 0.16365 |
| Rwork | 0.161 |
| R-free | 0.22285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qv9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.027 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 38483 | |
| Completeness [%] | 93.7 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
