4KRW
Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 41.137, 41.137, 72.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.630 - 2.010 |
| R-factor | 0.2272 |
| Rwork | 0.218 |
| R-free | 0.31444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.126 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.630 |
| High resolution limit [Å] | 2.010 |
| Completeness [%] | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 0.2 M sodium iodide, 20% (v/v) PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
