4KRU
X-ray structure of catalytic domain of endolysin from clostridium perfringens phage phiSM101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-09-27 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.770, 57.620, 73.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.960 - 1.370 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jfx |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.960 | 1.420 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.024 | 0.071 |
Number of reflections | 42675 | |
<I/σ(I)> | 42.5 | 11.7 |
Completeness [%] | 98.4 | 85 |
Redundancy | 6.15 | 2.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 20% PEG 3000, 0.2M Lithium sulphate, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |