4KRO

Nanobody/VHH domain EgA1 in complex with the extracellular region of EGFR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	P 1 21 1
Unit cell lengths	66.207, 96.288, 128.097
Unit cell angles	90.00, 100.68, 90.00

Refinement procedure

Resolution	38.193 - 3.054
R-factor	0.2235
Rwork	0.221
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1YY9 AND 4KRN
RMSD bond length	0.005
RMSD bond angle	0.897
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	3.050	8.270	3.050
Rmerge	0.099	0.030	0.463
Number of reflections	29807
<I/σ(I)>	10.2
Completeness [%]	98.8	92.3	99.6
Redundancy	2.3	2.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	17.5% PEG3350, 1.5 M sodium chloride, 5% glycerol, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K