4KRN
Nanobody/VHH domain EgA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.980 |
Spacegroup name | P 43 |
Unit cell lengths | 52.769, 52.769, 62.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.960 - 1.553 |
R-factor | 0.2004 |
Rwork | 0.199 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i3v |
RMSD bond length | 0.007 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.357 | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmerge | 0.037 | 0.025 | 0.283 |
Number of reflections | 24694 | ||
<I/σ(I)> | 19 | ||
Completeness [%] | 99.2 | 98.5 | 94.5 |
Redundancy | 4.4 | 4.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG3350, 0.2 M ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |