4KRF
Structure of Human Argonaute-1 let-7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.390, 97.730, 72.700 |
| Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
| Resolution | 40.568 - 2.101 |
| R-factor | 0.1772 |
| Rwork | 0.175 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.723 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 | |
| High resolution limit [Å] | 2.100 | 9.400 | 2.100 |
| Rmerge | 0.041 | 0.022 | 0.320 |
| Number of reflections | 50618 | 625 | 2558 |
| <I/σ(I)> | 16.85 | 44.3 | 2.43 |
| Completeness [%] | 94.2 | 97.5 | 64.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 13% PEG 3350, 9% iso-propanol, 0.1M Tris pH 9.0, vapor diffusion, hanging drop, temperature 291K |
