4KR5

Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	C 1 2 1
Unit cell lengths	88.691, 89.123, 59.477
Unit cell angles	90.00, 95.58, 90.00

Refinement procedure

Resolution	19.890 - 1.500
R-factor	0.1456
Rwork	0.143
R-free	0.18590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.040	0.489
Number of reflections	72578
<I/σ(I)>	1.7	1.7
Completeness [%]	98.9	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	281	40% PEG 6000, 0.1M SODIUM ACETATE BUFFER, 200MM MGCL2, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 281K