4KQT
Crystal structure of a putative outer membrane chaperone (OmpH-like) (CC_1914) from Caulobacter crescentus CB15 at 2.83 A resolution (PSI Community Target, Shapiro)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97917,0.91837,0.97862 |
Spacegroup name | H 3 2 |
Unit cell lengths | 109.849, 109.849, 163.704 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.677 - 2.830 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.24520 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.019 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.677 | 45.677 | 2.930 |
High resolution limit [Å] | 2.830 | 6.050 | 2.830 |
Rmerge | 0.074 | 0.025 | 0.946 |
Number of reflections | 9290 | 1008 | 903 |
<I/σ(I)> | 16.65 | 45.6 | 1.9 |
Completeness [%] | 99.7 | 99.3 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.20M sodium chloride, 40.00% polyethylene glycol 400, 0.1M Na/K phosphate pH 6.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |