4KQP
Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in complex with glutamine at 0.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.990, 51.690, 44.230 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 44.200 - 0.950 |
| R-factor | 0.1149 |
| Rwork | 0.115 |
| R-free | 0.11970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.042 | 0.380 |
| Number of reflections | 119821 | |
| <I/σ(I)> | 19.8 | 3.24 |
| Completeness [%] | 98.5 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 281 | 30% PEG 6000, 100MM CACL2, 0.1M SODIUM ACETATE BUFFER, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
