4KQD
The crystal Structure of the N-terminal PAS domain of the F plasmid TraJ
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11584, 0.979566, 0.953697, 0.979685 |
| Spacegroup name | P 61 |
| Unit cell lengths | 95.228, 95.228, 102.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.660 - 1.550 |
| R-factor | 0.16116 |
| Rwork | 0.160 |
| R-free | 0.17799 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.470 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.058 | 0.708 |
| Number of reflections | 75754 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.8 | 97.2 |
| Redundancy | 10.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 2M Ammonium sulphate, 0.1 M sodium citrate, 5% glycerol, 5 mM DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
