4KQB

crystal structure of the Golgi casein kinase with Mn/ADP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	58.490, 101.702, 158.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.428 - 3.045
R-factor	0.2171
Rwork	0.215
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.844
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.428
High resolution limit [Å]	3.045
Number of reflections	18134

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	12% PEG4000, 200 mM imidazole-malate (4:1), 3 mM NiCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K