4KQ4
Crystal structure of Anti-IL-17A antibody CNTO7357
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-02-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 65.500, 65.500, 99.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.450 |
| R-factor | 0.21281 |
| Rwork | 0.210 |
| R-free | 0.27903 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ztx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.520 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.058 | 0.492 |
| Number of reflections | 17241 | |
| <I/σ(I)> | 25.3 | 4.3 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 18% PEG8000, cryoprotectant: 0.1 M HEPES, pH 7.5, 20% PEG8000, 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
