4KPP
Crystal Structure of H+/Ca2+ Exchanger CAX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.954, 97.386, 71.233 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 30.712 - 2.300 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.722 |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 33675 |
| Completeness [%] | 87.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 10% pentaerythritol propoxylate(5/4 PO/OH), 100mM MES-NaOH, 100mM NH4Cl, 10mM CaCl2, pH 6.5, vapor diffusion, temperature 293K |
