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4KOL

The structure of hemagglutinin from avian-origin H7N9 influenza virus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2013-04-25
DetectorADSC QUANTUM 315r
Wavelength(s)1.0000
Spacegroup nameH 3 2
Unit cell lengths116.898, 116.898, 296.588
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution39.085 - 2.799
R-factor0.233
Rwork0.230
R-free0.28250
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.011
RMSD bond angle1.554
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.8_1069))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.900
High resolution limit [Å]2.7992.800
Number of reflections19421
Completeness [%]98.9100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.529116% w/v PEG 3350, 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

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