4KNY
Crystal structure of the response regulator KdpE complexed to DNA in an active-like conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 133.140, 133.140, 133.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.570 - 2.945 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zh4 3zq7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.763 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1352)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.570 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 25980 | |
| <I/σ(I)> | 12.1 | 3.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 285 | 10-15% PEG 4K, 0.1 M sodium acetate buffer (pH 5.5), 0.1 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
