4KNV
The crystal structure of APO HUMAN HDHD4 FROM SE-MAD
Experimental procedure
| Experimental method | MULTIPLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97821,0.97971,0.95370 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.340, 53.436, 63.958 |
| Unit cell angles | 65.50, 74.99, 85.17 |
Refinement procedure
| Resolution | 33.360 - 1.993 |
| R-factor | 0.2011 |
| Rwork | 0.200 |
| R-free | 0.24580 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 ((DENZO)) |
| Data scaling software | HKL-2000 ((SCALEPACK)) |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.993 | 2.000 |
| Rmerge | 0.073 | 0.200 |
| Number of reflections | 33961 | |
| <I/σ(I)> | 17.2 | 6.4 |
| Completeness [%] | 92.2 | 58.1 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1.6 M sodium/postassium phosphate, pH 5.6, vapor diffusion, hanging drop, temperature 277K |
