4KNU
Copper nitrite reductase from Nitrosomonas europaea at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.3776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.087, 136.940, 121.486 |
| Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.800 |
| R-factor | 0.17297 |
| Rwork | 0.171 |
| R-free | 0.20905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 142783 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 5% glycerol, 20% PEG 3350, 0.7-1.05 mM LiCl, and 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
