4KNU
Copper nitrite reductase from Nitrosomonas europaea at pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Collection date | 2011-07-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.3776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.087, 136.940, 121.486 |
Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
Resolution | 29.800 - 1.800 |
R-factor | 0.17297 |
Rwork | 0.171 |
R-free | 0.20905 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.244 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.800 |
High resolution limit [Å] | 1.800 |
Number of reflections | 142783 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 5% glycerol, 20% PEG 3350, 0.7-1.05 mM LiCl, and 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP |