4KNH
Structure of the Chaetomium thermophilum adaptor nucleoporin Nup192 N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.874, 102.874, 443.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.766 - 2.702 |
R-factor | 0.1935 |
Rwork | 0.192 |
R-free | 0.23120 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.569 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 66696 | |
<I/σ(I)> | 21.6 | 3.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.9 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 294 | 6% PEG4000, 0.1 M MES, pH 5.7, 0.6 M magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |