4KN8
Crystal structure of Bs-TpNPPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Detector technology | CCD |
Collection date | 2012-12-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97914 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.802, 83.268, 175.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.171 - 1.502 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ift |
RMSD bond length | 0.013 |
RMSD bond angle | 1.454 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1186) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.802 |
High resolution limit [Å] | 1.500 |
Number of reflections | 91966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES sodium, 2.0M (NH4)2SO4, 3%(w/v) polyethylene glycol 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |