4KMT
Crystal structure of human germline antibody 5-51/O12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-05-05 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.670, 73.830, 103.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.17316 |
| Rwork | 0.171 |
| R-free | 0.21989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QOT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.021 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.278 |
| Number of reflections | 27026 | |
| <I/σ(I)> | 16.8 | 5.7 |
| Completeness [%] | 93.6 | 89.8 |
| Redundancy | 4.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 1.8 M ammonium sulfate, 0.1 M CHES, pH 9.5, 5% dioxane, cryoprotectant: 2.1 M ammonium sulfate, 0.1 M CHES, pH 9.5, 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
