4KMF
Crystal structure of Zalpha domain from Carassius auratus PKZ in complex with Z-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.367, 49.471, 29.585 |
| Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
| Resolution | 27.460 - 1.700 |
| R-factor | 0.1729 |
| Rwork | 0.170 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.602 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.061 | 0.035 | 0.325 |
| Number of reflections | 8549 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 97.5 | 90 | 89.9 |
| Redundancy | 3.4 | 2.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 30% PEG1500, 15 mM MnCl2, vapor diffusion, temperature 295K |
