4KM6
Human folate receptor alpha (FOLR1) at acidic pH, orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-02 |
Detector | NOIR-1 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.519, 63.339, 71.169 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.491 - 1.550 |
R-factor | 0.171 |
Rwork | 0.168 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.163 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 4.210 | 1.550 |
Rmerge | 0.087 | 0.038 | 0.495 |
Number of reflections | 24336 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 98.3 | 98.6 | 86.8 |
Redundancy | 5.7 | 5.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 12 mg/mL co-purified with methotrexate in 0.1 M sodium citrate, , 35 % (v/v) jeffamine ED-2001 (MTX not present in structure), pH 5.5, Vapor diffusion, sitting drop, temperature 293K |