4KM3
Discovery of a novel structural motif in methionine aminopeptidase from Streptococci with possible post-translational modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-03 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 65 |
| Unit cell lengths | 109.687, 109.687, 164.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.750 - 3.200 |
| R-factor | 0.2138 |
| Rwork | 0.210 |
| R-free | 0.28493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tb5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.750 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 18507 | |
| <I/σ(I)> | 14.7 | 2.493 |
| Completeness [%] | 91.6 | 91.64 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 0.056 M Sodium phosphate monobasic monohydrate, 1.344M Potassium phosphate dibasic , pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
