4KLO
DNA polymerase beta matched nick complex with Mg2+ and PPi, 30 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-13 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.814, 79.966, 55.380 |
Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
Resolution | 22.616 - 1.845 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.011 |
RMSD bond angle | 1.091 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 | 1.920 |
High resolution limit [Å] | 1.845 | 2.330 | 1.845 |
Rmerge | 0.083 | 0.114 | 0.117 |
Number of reflections | 34639 | ||
<I/σ(I)> | 33.4 | 9.32 | 5.659 |
Completeness [%] | 95.5 | 99.2 | 79.1 |
Redundancy | 3.2 | 3.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |