4KLL

DNA polymerase beta matched product complex with Mg2+, 45 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-29
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	50.423, 79.570, 55.226
Unit cell angles	90.00, 107.69, 90.00

Refinement procedure

Resolution	23.758 - 1.841
R-factor	0.1819
Rwork	0.179
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
RMSD bond length	0.010
RMSD bond angle	1.088
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.841	1.841
Rmerge	0.061	0.297
Number of reflections	33832
<I/σ(I)>	23	2.363
Completeness [%]	94.3	80.9
Redundancy	2.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K