4KLJ
DNA polymerase beta matched product complex with Mg2+, 5 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.000, 80.600, 55.500 |
Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
Resolution | 31.988 - 1.799 |
R-factor | 0.1834 |
Rwork | 0.179 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.012 |
RMSD bond angle | 1.084 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.799 | 1.799 |
Rmerge | 0.071 | 0.332 |
Number of reflections | 38068 | |
<I/σ(I)> | 15.3 | 2.87 |
Completeness [%] | 96.9 | 73.7 |
Redundancy | 3.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |