4KLH

DNA polymerase beta matched product complex with Mn2+, 40 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-10
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	50.900, 80.200, 55.100
Unit cell angles	90.00, 107.90, 90.00

Refinement procedure

Resolution	25.089 - 1.876
R-factor	0.2109
Rwork	0.205
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
RMSD bond length	0.012
RMSD bond angle	1.114
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.230	1.950
High resolution limit [Å]	1.876	2.120	1.876
Rmerge	0.075	0.217	0.516
Number of reflections	33530
<I/σ(I)>	19.4	5.18	2.039
Completeness [%]	97.3	98.6	88.6
Redundancy	4.6	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K