4KLF
DNA polymerase beta matched reactant complex with Mg2+, 20 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-09 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.750, 79.956, 55.320 |
Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
Resolution | 24.198 - 1.850 |
R-factor | 0.1842 |
Rwork | 0.179 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.017 |
RMSD bond angle | 1.124 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 | 1.920 |
High resolution limit [Å] | 1.850 | 2.330 | 1.850 |
Rmerge | 0.053 | 0.113 | 0.318 |
Number of reflections | 35045 | ||
<I/σ(I)> | 25.2 | 10.8 | 2.778 |
Completeness [%] | 97.3 | 100 | 87.9 |
Redundancy | 3.8 | 4 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |