4KL7
Crystal structure of the catalytic domain of RpfB from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | Saturn944 |
Wavelength(s) | 1.5 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.427, 51.595, 66.594 |
Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
Resolution | 25.327 - 1.450 |
R-factor | 0.14727 |
Rwork | 0.145 |
R-free | 0.19221 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.531 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.450 |
Number of reflections | 38135 |
Completeness [%] | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 6 mg mL-1, 0,5% (w/v) PEG8000, 1M ammonium sulphate, 0,1 M Hepes, pH 7.5, and 120 mM benzamidine , VAPOR DIFFUSION, HANGING DROP, temperature 293K |