4KKY
Crystal structure of N-(1-Pyrene)acetamide labeled P450cam in substrate bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-01-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.950, 101.990, 57.350 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 33.270 - 2.000 |
| R-factor | 0.16992 |
| Rwork | 0.170 |
| R-free | 0.22532 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.403 |
| Data reduction software | MOSFLM |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.273 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 27318 |
| Completeness [%] | 73.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287.15 | 20% PEG 8000, 0.2M potassium Chloride, 0.05M Bis-tris Buffer. 1mM camphor., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 287.15K |
