4KKQ

Crystal structure of Vibrio cholerae RbmA (crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9771
Spacegroup name	P 41 21 2
Unit cell lengths	117.267, 117.267, 112.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.508 - 2.160
R-factor	0.1817
Rwork	0.180
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.160	2.160
Number of reflections	45502
Completeness [%]	99.9	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1 M BIS-TRIS, 10% Polyethylene glycol 10,000, 25% Xylitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K