4KKP
Crystal structure of Vibrio cholerae RbmA (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.375, 119.375, 105.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.440 - 2.500 |
| R-factor | 0.1726 |
| Rwork | 0.169 |
| R-free | 0.21350 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 26787 | |
| Completeness [%] | 99.5 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.1 M BIS-TRIS, 3.0 M NaCl, 25% Xylitol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
