4KKB
Structure of the E148A mutant of CLC-ec1 deltaNC construct in 20mM fluoride and 20mM Bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.718, 97.533, 170.568 |
| Unit cell angles | 90.00, 131.75, 90.00 |
Refinement procedure
| Resolution | 29.734 - 3.021 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.020 |
| Number of reflections | 107647 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 26% PEG400, 100mM Na/K tartrate, 50mM hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
