4KJZ
Crystal Structure of Thermus Thermophilus IF2, Apo and GDP-bound Forms (2-474)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97923 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.830, 177.816, 61.793 |
Unit cell angles | 90.00, 89.87, 90.00 |
Refinement procedure
Resolution | 46.760 - 2.800 |
R-factor | 0.24138 |
Rwork | 0.240 |
R-free | 0.27564 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b3x |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.010 | 2.900 |
High resolution limit [Å] | 2.800 | 3.530 | 2.800 |
Number of reflections | 63086 | ||
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 292 | 40 mM sodium cacodylate, pH 5.4, 100 mM calcium acetate, 10 mM glycyl-glycine, 10 mM taurine, 8% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 292K |