4KJR
Crystal structure of selenium substituted Ca2+/H+ antiporter proteinYfkE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-13 |
Detector | NOIR-1 |
Wavelength(s) | 0.9790, 0.9792, 0.9641 |
Spacegroup name | H 3 |
Unit cell lengths | 170.297, 170.297, 95.265 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.160 - 3.000 |
R-factor | 0.24046 |
Rwork | 0.239 |
R-free | 0.26809 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 85.160 |
High resolution limit [Å] | 2.900 |
Number of reflections | 22530 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 23-25% PEG400, 0.2M Ammonium Sulfate, 20mM NaCl, 0.1M NaAc pH4.0, 3% Hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |