4KJP
Structure of the CLC-ec1 deltaNC construct in the absence of halide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.449, 98.246, 171.026 |
Unit cell angles | 90.00, 132.08, 90.00 |
Refinement procedure
Resolution | 24.887 - 3.200 |
R-factor | 0.2243 |
Rwork | 0.222 |
R-free | 0.26510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.825 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 47173 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 23% PEG400, 100mM K-isethionate, 100 mM Glycine , pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |