4KJP
Structure of the CLC-ec1 deltaNC construct in the absence of halide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.449, 98.246, 171.026 |
| Unit cell angles | 90.00, 132.08, 90.00 |
Refinement procedure
| Resolution | 24.887 - 3.200 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 47173 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 23% PEG400, 100mM K-isethionate, 100 mM Glycine , pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
