4KJO
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.040, 60.402, 38.643 |
| Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
| Resolution | 38.510 - 1.900 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.913 |
| Data reduction software | APEX |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.028 | 0.256 |
| Number of reflections | 11300 | |
| <I/σ(I)> | 24.06 | 3.71 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.46 | 8.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
