4KJG

Structure of Rat Intestinal Alkaline Phosphatase expressed in insect cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-14
Detector	RAYONIX MX300HS
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	90.306, 167.170, 71.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.420 - 2.380
R-factor	0.1663
Rwork	0.164
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.11.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.380	5.130	2.380
Rmerge	0.171	0.079	0.574
Number of reflections	44083
<I/σ(I)>	5.7
Completeness [%]	99.9	99.9	100
Redundancy	5.6	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	1.4 M Sodium citrate, 0.1 M Hepes, pH 7.5, Vapor diffusion, temperature 293K