4KJG
Structure of Rat Intestinal Alkaline Phosphatase expressed in insect cell
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-14 |
Detector | RAYONIX MX300HS |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.306, 167.170, 71.843 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.420 - 2.380 |
R-factor | 0.1663 |
Rwork | 0.164 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.470 |
High resolution limit [Å] | 2.380 | 5.130 | 2.380 |
Rmerge | 0.171 | 0.079 | 0.574 |
Number of reflections | 44083 | ||
<I/σ(I)> | 5.7 | ||
Completeness [%] | 99.9 | 99.9 | 100 |
Redundancy | 5.6 | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 1.4 M Sodium citrate, 0.1 M Hepes, pH 7.5, Vapor diffusion, temperature 293K |