4KIA
Crystal structure of LmHde, heme-degrading enzyme, from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.610, 59.474, 45.434 |
| Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
| Resolution | 45.290 - 1.750 |
| R-factor | 0.19101 |
| Rwork | 0.188 |
| R-free | 0.24432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fez |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.165 | |
| Number of reflections | 14209 | |
| <I/σ(I)> | 30.41 | 5.78 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 287 | 25% PEG 3350, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 5.6, MICROBATCH, temperature 287K |
