4KI2

Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA

Replaces:  4GOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	CCD
Collection date	2009-04-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 62 2 2
Unit cell lengths	118.110, 118.110, 200.845
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.530 - 3.300
R-factor	0.2163
Rwork	0.212
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	13060
<I/σ(I)>		1.8
Completeness [%]	100.0	100
Redundancy	14.4	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295.15	100 mM Tris, pH 8.5, 5% w/v PEG8000, 20% w/v PEG300, 10% v/v glycerol, 0.15% w/v mellitic acid, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K