4KI0
Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07216 |
Spacegroup name | P 1 |
Unit cell lengths | 81.592, 96.631, 112.321 |
Unit cell angles | 85.30, 79.11, 73.00 |
Refinement procedure
Resolution | 19.820 - 2.380 |
R-factor | 0.19387 |
Rwork | 0.192 |
R-free | 0.22826 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rlf |
RMSD bond length | 0.005 |
RMSD bond angle | 1.063 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 122805 | |
Completeness [%] | 95.9 | 86.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 27% poly-ethylene glycol 400, 200 mM NaCl, 50 mM MgCl2, 100 mM sodium HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |