4KHS

Ternary complex of RB69 mutant L415F with a ribonucleotide at 0 position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2010-04-09
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	80.699, 119.572, 127.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.077 - 2.120
R-factor	0.1801
Rwork	0.176
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.815
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((PHENIX.REFINE: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	66285
Completeness [%]	99.9	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	50 MM TRIS-HCL, 10% PEG350, 220 MM CALCIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K, pH 7.5, temperature 298K