4KHO

Structure of the FACT complex Subunit Spt16M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	61.550, 75.030, 142.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.143 - 2.000
R-factor	0.209
Rwork	0.208
R-free	0.23570
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.800
High resolution limit [Å]	2.000
Number of reflections	52572
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	6% PEG 8000, 0.1M Na-cacodylate pH 5.5, 0.2M Ca-acetate hydrate, VAPOR DIFFUSION, temperature 298.0K