4KHB
Structure of the Spt16D Pob3N heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.391, 128.082, 132.329 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.778 - 2.400 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.24350 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.912 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.800 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 57453 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | 2.2M NH4SO4, 0.2M Na-K-tatrate, 0.2M Na3citrate pH 5.6, VAPOR DIFFUSION, temperature 298.0K |