4KH8
Crystal structure of a Lipocalin-like protein (EF0376) from Enterococcus faecalis V583 at 1.60 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97903,0.97871 |
Spacegroup name | P 65 |
Unit cell lengths | 99.745, 99.745, 56.956 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.046 - 1.600 |
R-factor | 0.1424 |
Rwork | 0.141 |
R-free | 0.17270 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.533 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.046 | 27.046 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.073 | 0.681 | |
Total number of observations | 2934 | 20074 | |
Number of reflections | 42670 | ||
<I/σ(I)> | 10.5 | 7.9 | 1.1 |
Completeness [%] | 99.9 | 98.2 | 100 |
Redundancy | 6.2 | 5.9 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 12.0% Glycerol, 1.5M ammonium sulfate, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |