4KGK

Bacterial tRNA(HIS) Guanylyltransferase (Thg1)-Like Protein in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-26
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.03
Spacegroup name	P 41
Unit cell lengths	111.254, 111.254, 129.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.410 - 2.950
R-factor	0.2105
Rwork	0.208
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.637
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.070
High resolution limit [Å]	2.950	2.960
Rmerge	0.096
Number of reflections	30738
Completeness [%]	92.7	80.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	13% (w/v) polyethylene glycol 4000, 50 mM MgSO4 and 100 mM Tris(2-carboxyethyl) phosphine, pH 7 and 2 mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 291K