4KGH
Crystal Structure of human splunc1 lacking the secretion signal sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-20 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.475, 203.727, 118.612 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.639 - 2.806 |
R-factor | 0.2507 |
Rwork | 0.246 |
R-free | 0.29520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.854 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.600 | 2.800 |
Rmerge | 0.092 | 0.053 | 0.379 |
Number of reflections | 13819 | ||
<I/σ(I)> | 12 | ||
Completeness [%] | 95.8 | 98.9 | 72.5 |
Redundancy | 6.5 | 6.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 8% PEG 400, .2 M tri-sodium citrate, .1 M Tris-HCl pH 8.5, vapor diffusion, temperature 298K |